SrasV1, Main, Exploration, bibRecord, 004D54

Subcellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein

Identifieur interne : 004D54 ( Main/Exploration ); précédent : 004D53; suivant : 004D55

Subcellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein

Auteurs : Jaehwan You [Royaume-Uni] ; Brian K. Dove [Royaume-Uni] ; Luis Enjuanes [Espagne] ; Marta L. Dediego [Espagne] ; Enrique Alvarez [Espagne] ; Gareth Howell [Royaume-Uni] ; Paul Heinen [Royaume-Uni] ; Maria Zambon [Royaume-Uni] ; Julian A. Hiscox [Royaume-Uni]

Source :

Journal of general virology [ 0022-1317 ] ; 2005.

RBID : Pascal:06-0028823

Descripteurs français

KwdFr :
- Animaux, Biologie informatique, Cellules Vero, Cytoplasme (), Données de séquences moléculaires, Délétion de séquence, Microscopie confocale, Nucléole (), Protéines nucléocapside (), Protéines nucléocapside (analyse), Protéines nucléocapside (génétique), Signaux de localisation nucléaire, Similitude de séquences, Structure tertiaire des protéines, Séquence d'acides aminés, Virus du SRAS (génétique), Virus du SRAS (physiologie).
MESH :
- analyse : Protéines nucléocapside.
- génétique : Protéines nucléocapside, Virus du SRAS.
- physiologie : Virus du SRAS.
Pascal (Inist)
- Animaux, Biologie informatique, Cellules Vero, Coronavirus, Cytoplasme, Données de séquences moléculaires, Délétion de séquence, Localisation, Microscopie confocale, Nucléocapside, Nucléole, Protéine, Microbiologie, Protéines nucléocapside, Signaux de localisation nucléaire, Similitude de séquences, Structure tertiaire des protéines, Séquence d'acides aminés, Virologie, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Amino Acid Sequence, Animals, Cell Nucleolus (chemistry), Chlorocebus aethiops, Computational Biology, Coronavirus, Cytoplasm (chemistry), Localization, Microbiology, Microscopy, Confocal, Molecular Sequence Data, Nuclear Localization Signals, Nucleocapsid, Nucleocapsid Proteins (analysis), Nucleocapsid Proteins (chemistry), Nucleocapsid Proteins (genetics), Protein, Protein Structure, Tertiary, SARS Virus (genetics), SARS Virus (physiology), Sequence Deletion, Sequence Homology, Severe acute respiratory syndrome, Vero Cells, Virology.
MESH :
- chemical , analysis : Nucleocapsid Proteins.
- chemical , chemistry : Nucleocapsid Proteins.
- chemical , genetics : Nucleocapsid Proteins.
- chemical : Nuclear Localization Signals.
- chemistry : Cell Nucleolus, Cytoplasm.
- genetics : SARS Virus.
- physiology : SARS Virus.
- Amino Acid Sequence, Animals, Chlorocebus aethiops, Computational Biology, Microscopy, Confocal, Molecular Sequence Data, Protein Structure, Tertiary, Sequence Deletion, Sequence Homology, Vero Cells.

Abstract

The coronavirus nucleocapsid (N) protein is a viral RNA-binding protein with multiple functions in terms of virus replication and modulating cell signalling pathways. N protein is composed of three distinct regions containing RNA-binding motif(s), and appropriate signals for modulating cell signalling. The subcellular localization of severe acute respiratory syndrome coronavirus (SARS-CoV) N protein was studied. In infected cells, SARS-CoV N protein localized exclusively to the cytoplasm. In contrast to the avian coronavirus N protein, overexpressed SARS-CoV N protein remained principally localized to the cytoplasm, with very few cells exhibiting nucleolar localization. Bioinformatic analysis and deletion mutagenesis coupled to confocal microscopy and live-cell imaging, revealed that SARS-CoV N protein regions I and III contained nuclear localization signals and region II contained a nucleolar retention signal. However, cytoplasmic localization was directed by region III and was the dominant localization signal in the protein.

Affiliations:

Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Cell Nucleolus (chemistry)</term>
<term>Chlorocebus aethiops</term>
<term>Computational Biology</term>
<term>Coronavirus</term>
<term>Cytoplasm (chemistry)</term>
<term>Localization</term>
<term>Microbiology</term>
<term>Microscopy, Confocal</term>
<term>Molecular Sequence Data</term>
<term>Nuclear Localization Signals</term>
<term>Nucleocapsid</term>
<term>Nucleocapsid Proteins (analysis)</term>
<term>Nucleocapsid Proteins (chemistry)</term>
<term>Nucleocapsid Proteins (genetics)</term>
<term>Protein</term>
<term>Protein Structure, Tertiary</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (physiology)</term>
<term>Sequence Deletion</term>
<term>Sequence Homology</term>
<term>Severe acute respiratory syndrome</term>
<term>Vero Cells</term>
<term>Virology</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Biologie informatique</term>
<term>Cellules Vero</term>
<term>Cytoplasme ()</term>
<term>Données de séquences moléculaires</term>
<term>Délétion de séquence</term>
<term>Microscopie confocale</term>
<term>Nucléole ()</term>
<term>Protéines nucléocapside ()</term>
<term>Protéines nucléocapside (analyse)</term>
<term>Protéines nucléocapside (génétique)</term>
<term>Signaux de localisation nucléaire</term>
<term>Similitude de séquences</term>
<term>Structure tertiaire des protéines</term>
<term>Séquence d'acides aminés</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (physiologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Nucleocapsid Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Nucleocapsid Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Nuclear Localization Signals</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Protéines nucléocapside</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Cell Nucleolus</term>
<term>Cytoplasm</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines nucléocapside</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Chlorocebus aethiops</term>
<term>Computational Biology</term>
<term>Microscopy, Confocal</term>
<term>Molecular Sequence Data</term>
<term>Protein Structure, Tertiary</term>
<term>Sequence Deletion</term>
<term>Sequence Homology</term>
<term>Vero Cells</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Animaux</term>
<term>Biologie informatique</term>
<term>Cellules Vero</term>
<term>Coronavirus</term>
<term>Cytoplasme</term>
<term>Données de séquences moléculaires</term>
<term>Délétion de séquence</term>
<term>Localisation</term>
<term>Microscopie confocale</term>
<term>Nucléocapside</term>
<term>Nucléole</term>
<term>Protéine</term>
<term>Microbiologie</term>
<term>Protéines nucléocapside</term>
<term>Signaux de localisation nucléaire</term>
<term>Similitude de séquences</term>
<term>Structure tertiaire des protéines</term>
<term>Séquence d'acides aminés</term>
<term>Virologie</term>
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The coronavirus nucleocapsid (N) protein is a viral RNA-binding protein with multiple functions in terms of virus replication and modulating cell signalling pathways. N protein is composed of three distinct regions containing RNA-binding motif(s), and appropriate signals for modulating cell signalling. The subcellular localization of severe acute respiratory syndrome coronavirus (SARS-CoV) N protein was studied. In infected cells, SARS-CoV N protein localized exclusively to the cytoplasm. In contrast to the avian coronavirus N protein, overexpressed SARS-CoV N protein remained principally localized to the cytoplasm, with very few cells exhibiting nucleolar localization. Bioinformatic analysis and deletion mutagenesis coupled to confocal microscopy and live-cell imaging, revealed that SARS-CoV N protein regions I and III contained nuclear localization signals and region II contained a nucleolar retention signal. However, cytoplasmic localization was directed by region III and was the dominant localization signal in the protein.</div>
</front>
</TEI>
<affiliations><list><country><li>Espagne</li>
<li>Royaume-Uni</li>
</country>
<region><li>Angleterre</li>
<li>Communauté de Madrid</li>
<li>Yorkshire-et-Humber</li>
</region>
<settlement><li>Leeds</li>
</settlement>
<orgName><li>Université de Leeds</li>
</orgName>
</list>
<tree><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="You, Jaehwan" sort="You, Jaehwan" uniqKey="You J" first="Jaehwan" last="You">Jaehwan You</name>
</region>
<name sortKey="Dove, Brian K" sort="Dove, Brian K" uniqKey="Dove B" first="Brian K." last="Dove">Brian K. Dove</name>
<name sortKey="Heinen, Paul" sort="Heinen, Paul" uniqKey="Heinen P" first="Paul" last="Heinen">Paul Heinen</name>
<name sortKey="Hiscox, Julian A" sort="Hiscox, Julian A" uniqKey="Hiscox J" first="Julian A." last="Hiscox">Julian A. Hiscox</name>
<name sortKey="Hiscox, Julian A" sort="Hiscox, Julian A" uniqKey="Hiscox J" first="Julian A." last="Hiscox">Julian A. Hiscox</name>
<name sortKey="Howell, Gareth" sort="Howell, Gareth" uniqKey="Howell G" first="Gareth" last="Howell">Gareth Howell</name>
<name sortKey="Zambon, Maria" sort="Zambon, Maria" uniqKey="Zambon M" first="Maria" last="Zambon">Maria Zambon</name>
</country>
<country name="Espagne"><region name="Communauté de Madrid"><name sortKey="Enjuanes, Luis" sort="Enjuanes, Luis" uniqKey="Enjuanes L" first="Luis" last="Enjuanes">Luis Enjuanes</name>
</region>
<name sortKey="Alvarez, Enrique" sort="Alvarez, Enrique" uniqKey="Alvarez E" first="Enrique" last="Alvarez">Enrique Alvarez</name>
<name sortKey="Dediego, Marta L" sort="Dediego, Marta L" uniqKey="Dediego M" first="Marta L." last="Dediego">Marta L. Dediego</name>
</country>
</tree>
</affiliations>
</record>

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Subcellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein

Subcellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein

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